eDNA Sampler - Environmental DNA Sampling

DNA SAMPLER - Environmental DNA Sampling

Environmental DNA (eDNA) sampling has revolutionised aquatic ecosystem monitoring by enabling the detection of genetic material shed by organisms into the environment. This non-invasive passive monitoring method allows for comprehensive biodiversity assessments without the need for direct observation, acoustic monitoring or capture.

Autonomous eDNA Multi-sampling for the Detection of Biological Genomic Signatures in Water

Our cutting-edge Autonomous eDNA Sampler is engineered to facilitate precise and efficient collection of eDNA samples in various aquatic environments. Key features include:

Automated Operation: Pre-programmed missions enable autonomous sampling, reducing the need for manual intervention and increasing operational efficiency.
Micro-Fluidic Architecture: This design minimizes reagent consumption and securely stores waste products for proper disposal, promoting environmental responsibility.
Sample Preservation: Utilizing RNAlater for sample preservation ensures the integrity of genetic material during long-term, unsupervised deployments.
Customizable Filter Materials: Various filter materials, such as polycarbonate and PVDF, are available to meet specific research requirements.
Optional Integrated Fluorometer: An optional 4-channel fluorometer (Chl-a, PC, PE, FDOM) provides real-time fluorescence measurements, adding valuable data layers.
Compact Design: Its portable form factor allows deployment from diverse platforms, including small boats and autonomous underwater vehicles.

Multi-sample eDNA Collection Made Easy

eDNA sampling offers a cost-effective and less invasive alternative to traditional biodiversity assessment methods. It facilitates early detection of invasive species, parasites, and pathogens, contributing to proactive environmental management.

eDNA sampling is a valuable tool for studying both prokaryotic and eukaryotic biodiversity in the water column.

Dartmouth Ocean Technologies has developed a portable, convenient solution for use in many data collection scenarios.

The programmable nature of the instrument allows for increased sampling resolution over long periods of time – ideal for time series studies.

DNA can be extracted from filters using various commercial kits (i.e. Qiagen, BioMeme). The integrated multi-sample holder makes transporting the samples more convenient.

Also available: the DOT eDNA Rapid Sampler

For survey operations requiring high-volume sample collection at speed, the DOT eDNA Rapid Sampler offers flow rates of up to 600 mL per minute and a compact, reagent-free design suited to ROV and AUV payload bays. Compare both systems to find the right fit for your programme.

Environmental Monitoring Applications

Coastal and Offshore Site Assessments: Commissioning and decommissioning of various coastal and offshore work sites:
- Oil and Gas
- Wind farms
- Aquaculture

Targeted Organism Surveillance: Monitoring the environment for specific organisms of interest:
- Harmful algae (i.e. Alexandrium sp.)
- Parasites or pathogens
- Indicator species

Molecular Analysis Compatibility: Collected samples are compatible with downstream molecular techniques, including quantitative PCR (qPCR), for detailed genetic analysis.

Understanding how eDNA monitoring works in practice

For a technical overview of environmental DNA monitoring in marine environments, including integration considerations and operational limitations, see our eDNA Insights article.

eDNA Sampler Promotional Video

The Case of the Wayward Whales

Product Specifications

Weight:

Standard:
In Air: 10.1 kg (22.2 lbs)
In Salt Water: 0.53 kg (1.2 lbs)

Extra Capacity:
In Air: 10.2 kg (22.4 lbs)
In Salt Water: 0.53 kg (1.2 lbs)

Deep Water:
In Air: 10.3 kg (22.2 lbs)
In Salt Water: 0.54 kg (1.2 lbs)

Dimensions:

Diameter: 167 mm (6.6 in)
Length:
Standard: 721 mm (28.4 in)
Extra Capacity: 800 mm (31.5 in)
Deep Water: 800 mm (31.5 in)
+ compensator

Sample Filters: (defaults)

– Size: 25mm
– Pore Size: 0.2μm to 2.0μm
– Material: Polycarbonate (PC)

Sample Rate: 20 to 90 minutes per filter (volume dependent)

Sample Volume: Tested for 100 – 500 ml (user-definable)

Intake Filter: 35μm PEEK (default)

Data Output: RS232

Reagents: (volumes per filter) 5% HCl (14 ml) RNAlater (6 ml) Milli-Q Water (42 ml)

Depth Rating: 20 metres

Power Draw: 7VDC or 24VDC

Filters per Cartridge: 9

Options:

Filter composition and pore size can be changed to suit different needs
External battery case (Lithium primary)
Hard, floodable reagent case